Cylinder six by chris zabriskie is licensed under a creative commons attribution license source. This dual monochromatic, multimode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384well microplates. Spectramax m3, m4, m5, and m5 e multimode microplate. Spectramax gemini em microplate reader molecular devices. Spectramax m2 plate reader at the university of pennsylvania. With optimized reagents and the industry leading data acquisition and analysis tool, softmax pro 7 software, the spectramax to perfectly match your needs. Page 72 spectramax id3 multimode microplate reader user guide 3. Spectramax m series multimode microplate readers from. Molecular devices spectramax plus 384 microplate reader. It is able to find out in minutes the results of dozens of samples. To see the microplate chamber temperature, you must use softmax pro software. The molecular devices spectramax m5 and m2, and gemini xps and em are versatile microplate readers whose detection modes include fluorescence intensity.
Spectramax m2 and use thereof is covered by issued u. The plate is set up for reading and images are acquired according to specified parameters. Spectramax m2 plate reader is an eaglei resource of type microplate reader at the university of pennsylvania. Laboratory controls offers a best in the business full 90 day warranty on all refurbished items.
Data collection and display softmax pro collects and stores all raw data received from the instrument. Spectramax microplate spectrophotometer operators manual. Multi user multi reader control winkqcl 4 software introduced multithreaded software with the ability to control multiple readers from the same computer, at the same. Fluorescence readers biotek flx800 molecular devices gemini em, gemini xps and gemini xs multimode readers absorbance and fluorescence readers biotek synergy 2 molecular devices spectramax m2, m2e, m3, m4, m5 and m5e molecular devices flexstation. Page 9 pressing and holding either arrow increments or decrements the displayed temperature by 1c until it is released. Spectramax plus384 microplate spectrophotometer operators manual. Spectramax m2 plate reader microplate reader data and elisa data analysis does not get any, search among more than 1. The spectramax 190 reader can be integrated with molecular devices stakmax microplate stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The tecan infinite m200 is a user friendly and affordable multimode reader, designed to cater for the needs of todays applications. The spectramax m2 and m2e systems from molecular devices are multidetection microplate readers with dualmonochromators, dualmode cuvette ports, and top and bottomreading capability topreading only on the m2. Spectramax m2m2e user guide university of michigan. With advanced 8channel optics, you can analyze a plate in as little as 5 seconds.
Providing ultrafast, full spectral range detection for cuvettes, 96 and 384well microplates. Softmax pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. This document is provided to customers who have purchased molecular devices. This user guide describes features available in softmax pro software release 5. Control panel microplate drawer optical system back panel connections and power switch 1. For example, microplates containing different samp les can be read using the same or. Detection modalities include absorbance uvvis abs and fluorescence intensity fi. Uvvisible absorbance abs fluorescence intensity fi top read the spectramax m2 and m2e microplate readers are the standard for uvvisible multimode reader absorbance, providing ultrafast. Softmax pro software user guide marine biological laboratory. Uvvisible absorbanceabs fluorescence intensityfi top read the spectramax m2 microplate reader is the standard for uvvisible multimode reader absorbance. Spectramax 190 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax 190 control panel are shown in boxed helvetica type.
Spectramax m2m2e microplate readers molecular devices. For example, microplates containing different samples can be read using the same or. Data is displayed in a grid format that corresponds to the wells in a microplate all instruments or individual cuvettes using spectramax plus, plus384, m2, m2e, m5e or m5. Spectramax plus, plus384, m2, m2e, m5e or m5 readers.
Molecular devices spectramax plus 384 microplate reader gmi. Spectramax m2 and m2e multimode microplate readers, see page 121. The molecular devices spectramax m2e provides powerful multi detection reading for microplates and cuvettes in a flexible, dual monochromator, easy to use instrument. Microplate readers use a vertical light path so the distance of the light through the. Configurability is an important aspect of bioteks multimode readers to provide the most value for laboratory budgets. This benchmark for uvvisible microplate readers is capable of processing up to 384 samples in a single plate. Spectramax m2m2e multimode microplate readers, with softmax. The spectramax m2 and m2e multimode microplate readers provide two detection modes in one platform and offer the added functionality of bottomread fluorescence for cellbased assays. Analytical technologies group has deep experience maintaining and repairing molecular devices microplate readers, including the spectramax m series microplate readers m2, m2e, m3, m5, m5, and m5e.
Winkqcl 5 endotoxin detection and analysis software. Molecular devices spectramax m2e manuals manualslib. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Spectramax microplate reader user manual download page. Benefits user upgradeable application modules including cellular imaging sensitivity across spectrum with spectral fusion illumination expanded dynamic range with cooled pmt control and analytics provided by softmax pro software spectramax i3x the spectramax i3x from molecular devices is a multimode detection system that evolves with your future needs and offers an. After the plate is read, data will appear in the plate section. View online or download molecular devices spectramax m2e user manual. The infinite 200 can provide a full range of leading detection methods in one easytouse modular instrument, with quad4 monochromator. Spectramax m2 and m2e microplate reader user guide. Spectramax m series microplate readers, cuvette port, iq oq. Imaging with the spectramax minimax 300 imaging cytometer mirrors the plate reading workflow on the spectramax i3 x system.
Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Synergy and cytation readers offer modular features and outstanding specifications for performance and economy. The thermomax microplate reader provides thermal control and vertical shake, perfect for kinetic readings involving growth curves at 5 seconds per plate. Spectramaxabs absorbance microplate reader introduction. The molecular devices spectramax m3 multimode microplate reader covers a wide range of applications without the need to change filters. When a feature or capability applies to only certain readers, this exception is noted. Spectramax m2 microplate reader from molecular devices. Multimode microplate readers offer flexibility and ease of use over a broad range of applications. Laboratory controls will repair or replace your part. The spectramax m2 and m2e systems from molecular devices are multi detection microplate readers with dual monochromators, dualmode cuvette ports, and top and bottomreading capability topreading only on the m2. This reader also makes use of a unique temperatureindependent methodology for path length correction. These instruments have dual monochromators, so the optimization of excitation and emission wavelengths can easily be performed via spectral scanning without the purchase of additional.
Spectramax m series microplate readers, cuvette port, iq. Spectramax i3x molecular devices pdf catalogs technical. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. After your plate template has been constructed, you can print your plate. Click formula reference to view the softmax pro formula reference. Molecular devices spectramax 250 microplate reader manual. The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Components the main components of gemini readers described in this manual are. The control panel the control panel consists of a 2x20character lcd and eleven pressuresensitive membrane keys that can be used to control some functions of the instrument.
For a higher degree of automation, the automation vendor partners program has streamlined the integration of our microplate reader systems with all leading partner robots. Spectramax gemini em microplate reader a dualmonochromator spectrofluorometer system dual monochromators with the gemini em reader, users never have to worry about not having the right set of filters. Dual scanning microplate spectrofluorometer user guide. Molecular devices spectramax m2 user manual pdf download. Spectramax m3, m4, m5, and m5e multimode microplate.
Running both standard spectrophotometer and microplate reader applications at the same time is possible with the spectramax plus 384. This warranty covers your part against any material and or craftsmanship issues within one full year from purchase. Dec 16, 2020 spectramax microplate reader user manual download page. Spectramax m2 and m2e multimode microplate readers user guide. Spectramax and versamax plate readers operators manual 01120126 rev a 1. Jun 11, 2018 the new spectramax abs absorbance microplate readers are small but mighty. You cannot set a temperature beyond the upper 45c or lower 15c instrument limits.
Dual monochromators increase performance and assay flexibility the spectramax m series multimode microplate readers measure uv and visible absorbance, fluorescence, luminescence, fluorescence polarization, trf and htrf. Cylinder six by chris zabriskie is licensed under a creative commons attribution license. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Molecular devices spectramax m2 user manual and software guide. Fluorescence detector validation the spectratest abs1 absorbance plate is used to validate optical performance of spectramax, flexstation 3, and versamax microplate readers. Endpoint, kinetic, spectrum and areawell scanning read types and the pathcheck sensor allow. When a feature or capability applies to only certain readers. Molecular devices spectramax id3 user manual pdf download.
Gemini emxps dual scanning microplate spectrofluorometer user guide 01120128 rev. The system can perform readings in a photometric analysis mode as well as kinetic analysis at user configurable kinetic ranges and intervals, run onboard and temperature regulation diagnostics. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Touch plate name plate formattype settings to display the plate settings page where you can select the plate format and plate type. The plate name appears at the top center of the page.
Additionally, the spectramax m2 and m2e microplate readers can be seamlessly integrated with the stakmax microplate handling stacker through softmax pro software. Molecular devices spectramax 250 microplate reader manual warranty information laboratory controls offers a best in the business full 90 day warranty on all refurbished items. Molecular devices spectramax 250 microplate reader. Microplate readers include the m3, m4, m5, and m5e models. In this user guide, all references to spectramax multimode.
Spectramax m3, m4, m5, and m5e multimode microplate reader. The spectramax m5 multimode microplate reader delivers single mode reader performance in one multimode reader package. We routinely use this almost every day for various applications. Spectramaxabs absorbance microplate reader introduction youtube. Spectramax m3, m4, m5, and m5 e multimode microplate readers. Setup guide on the molecular devices spectramax m5m5e. Spectramax plus 384 microplate spectrophotometer operators manual introduction general overview the spectramax plus 384 microplate spectrophotometer provides rapid and sensitive uvvis measurements of a variety of analytes including speci. This manual focuses on the stakmax software application only and refers to the softmaxpro user guidefor specific details on overlapping functions.
In this user guide, all references to spectramax multimode microplate readers include the m3, m4, m5, and m5 e models. Service for spectramax m series multimode microplate readers. Reader documentation version no 8 oct 12 page 7 of 10 setup guide on the molecular devices spectramax m5m5e microplate reader have a question. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the spectramax microplate readers. All the members in our lab are highly impressed with this instrument. In this user guide, all references to spectramax multimode microplate readers include the m3, m4, m5, and m5e models. The m2e plate reader is a great choice for applications in dna, rna, and protein quantitation and purity checks, enzyme kinetics, drug dissolutions profiles, cytotoxicity assays, and many more. The spectrophotometric performance of the spectramax m2 and spectramax m2e is similar to the spectramax. Spectramax and versamax plate readers operators manual 01120126 rev a. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microp late or from the cuvette port. Cells in each image are identified by softmax pro software and cellbycell statistics are collected. Softmax pro user manual the remcho research group wiki.
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